Phos-tag(TM) SDS-PAGE相关产品
发布时间:2025-04-25 点击数:8

◆Reagents for Phos-tag™ SDS-PAGE gel preparation
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
30 w/v% Acrylamide/Bis Mixed solution(29:1) | 500 mL | 015-25635 | Ready-to-use"Solution A".30%T, 3.3%C |
Agarose H(High-strength type) | 1 g | 315-01203 | High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 - 1% and a separation range of 1 - 200 kbp. |
10 g | 319-01201 | ||
25 g | 317-01202 | ||
10% SDS Solution | 100 mL | 311-90271 | Ready-to-Use "Solution D" |
500 mL | 313-90275 | ||
Manganese(Ⅱ) Chloride Tetrahydrate, 99.0+ %(Titration) | 25 g | 134-15302 | for Molecular Biology Please use for preparation of "Solution F" |
100 g | 136-15301 | ||
Zinc Chloride | 25 g | 268-01902 | for Molecular Biology. Please use for preparation of "Solution M" |
Bis-Tris | 100 g | 345-04741 | Please use for preparation of "Solution N" |
Sodium Hydrogensulfite〈JIS Special Grade〉 | 25 g | 196-01372 | Please use for preparation of "Solution O" |
100 g | 198-01371 | ||
Sodium Hydrogensulfite 〈For Molecular Biology〉 | 100 g | 190-16461 | |
MOPS | 100 g | 345-01804 | Please use for preparation of "Solution P" |
250 g | 341-01801 | ||
MOPS〈For Molecular Biology〉 | 100 g | 341-08241 | |
500 g | 343-08245 | ||
Separating Gel Buffer Solution (x4) | 250 mL | 192-11041 | Ready-to-Use mixed solution of "Sol. B" and "Sol. D" for preparation of Resolving Gel. Contains SDS. |
Stacking Gel Buffer Solution (x4) | 250 mL | 199-11051 | Ready-to-Use mixed solution of "Sol.C" and "Sol. D" for preparation of Stacking Gel. Contains SDS. |
N,N,N',N'-Tetramethylethylenediamine (TEMED) | 25 mL | 205-06313 | for Electrophoresis |
10 w/v% Ammonium Peroxodisulfate Solution (Ammonium Persulfate Solution) | 25 mL | 019-15922 | Ready-to-Use "Solution G" |
◆Premixed Buffers
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
Running Buffer Solution (x10) | 1 L | 184-01291 | Ready-to-Use concentrated "Solution H" |
SDS-PAGE 10x Running Buffer | 1 L | 312-90321 | Ready-to-Use concentrated "Solution H" |
5 L | 318-90323 | ||
SDS-PAGE Buffer, pH 8.5 | 5 L | 192-16801 | Ready-to-Use "Solution H", 1×buffer. |
Tricine Running Buffer Solution (×10) | 1 L | 200-17071 | Composition: 0.5 M Tris/0.5 M Tricine/1% SDS |
Sample Buffer Solution (2ME+) (×2) | 25 mL | 196-11022 | Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol |
Sample Buffer Solution with 3-Mercapto-1,2-propanediol (×2) | 25 mL | 196-16142 | Laemmli Sample Buffer containing 3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME |
AquaBlot™ 10×High Efficiency Transfer Buffer | 1 L | 015-26213 | Can use it to transfer to a menbrane with high efficience. |
◆Enzyme for Dephosphorylation
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
Alkaline Phosphatase(for Biochemistry) | 50 U | 018-10693 | Applicable to dephosphorylation of proteins |
100 U | 012-10691 |
◆Reagents for Staining
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
Quick CBB Plus | 250 mL | 174-00553 | Ready-to-Use Sol. K. Fixing and destaining procedure are not required. No organic solvents are necessary. Protein bands are stained in 10 minutes. |
1 L | 178-00551 | ||
Quick-CBB ?Staining solution A: 1L x 1 ?Staining solution B: 1L x 1 | 2 L | 299-50101 | By mixing staing solution A and B, ready-to-Use Sol. K |
Silver Stain MS Kit | 20 tests | 299-58901 | Modified proteins by glycosilation and/or phosphorylation can be detected sub-nanogram level on the electrophoretic gel. |
Silver Stain Kit Wako | for 10 gels | 299-13841 | 50~100 times more sensitive than CBB method. |
Silver Stain Ⅱ Kit Wako | for 10 gels | 291-50301 | This kit contains Stopper, which can be adjusted the staining strength. |
Negative Gel Stain MS Kit | 20 tests | 293-57701 | Applicable to mass analysis and Western blot |
◆Protein Size Marker
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
WIDE-VIEW™ Prestained Protein Size Marker Ⅲ | 25 μL | 236-02463 | A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less distorted. |
500 μL | 230-02461 | ||
500 μL x3 | 234-02464 |
◆Positive Control(for confirmation of separation capacity of Phos-tag™ SDS-PAGE)
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
α-Casein, from BovineMilk, Dephosphorylated | 1 mg | 038-23221 | Mixture of phosphylated and dephosphorylated α-casein |
10 mg | 034-23223 |
◆Electrophoresis Apparatus Precast Gels
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
EasySeparator | 1 unit | 058-07681 | An electrophoresis tank for SuperSep precast polyacrylamide gels. |
SuperSep Ace, 6%, 13 wells | 10 gels | 195-15171 | Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation. Expired in 9 months after manufacture |
SuperSep Ace, 7.5%, 13 wells | 10 gels | 198-14941 | |
SuperSep Ace, 7.5%, 17 wells | 10 gels | 191-14931 | |
SuperSep Ace, 10%, 13 wells | 10 gels | 195-14951 | |
SuperSep Ace, 10%, 17 wells | 10 gels | 192-14961 | |
SuperSep Ace, 12.5%, 13 wells | 10 gels | 199-14971 | |
SuperSep Ace, 12.5%, 17 wells | 10 gels | 196-14981 | |
SuperSep Ace, 15%, 13 wells | 10 gels | 193-14991 | |
SuperSep Ace, 15%, 17 wells | 10 gels | 190-15001 |
◆Reagents for Western Blotting
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
ImmunoStar LD* ?Luminescence solution A ?Luminescence solution B | 200 cm2 | 296-69901 | Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate. Not available for sale in the US and Europe. |
1,000 cm2 | 292-69903 | ||
2,000 cm2 | 290-69904 | ||
ImmunoStar Zeta* | 200 cm2 | 291-72401 | Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal. |
1,000 cm2 | 297-72403 | ||
2,000 cm2 | 295-72404 | ||
ImmunoStar Basic* | 200 cm2 | 295-75101 | A cost-effective, stable and long-lasting product. |
2,000 cm2 | 291-75103 | ||
5,000 cm2 | 299-75104 | ||
Immuno Enhancer | 2 assays | 294-68601 | Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA |
10 assays | 290-68603 | ||
40 assays | 298-68604 |
* : Not available for sales in the US and Europe.
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