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Phos-tag(TM) SDS-PAGE相关产品

发布时间:2025-04-25 点击数:8
Phos-tag SDS-PAGE相关产品
 
◆Reagents for Phos-tag SDS-PAGE gel preparation
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
30 w/v% Acrylamide/Bis Mixed solution(29:1)
500 mL
015-25635
Ready-to-use"Solution A".30%T, 3.3%C
Agarose H(High-strength type)
1 g
315-01203
High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 - 1% and a separation range of 1 - 200 kbp.
10 g
319-01201
25 g
317-01202
10% SDS Solution
100 mL
311-90271
Ready-to-Use "Solution D"
500 mL
313-90275
Manganese(Ⅱ) Chloride Tetrahydrate,
             99.0+ %(Titration)
25 g
134-15302
for Molecular Biology Please use for preparation of "Solution F"
100 g
136-15301
Zinc Chloride
25 g
268-01902
for Molecular Biology. Please use for preparation of "Solution M"
Bis-Tris
100 g
345-04741
Please use for preparation of "Solution N"
Sodium Hydrogensulfite〈JIS   Special Grade〉
25 g
196-01372
Please use for preparation of "Solution O"
100 g
198-01371
Sodium Hydrogensulfite
             〈For Molecular Biology〉
100 g
190-16461
MOPS
100 g
345-01804
Please use for preparation of "Solution P"
250 g
341-01801
MOPS〈For Molecular Biology〉
100 g
341-08241
500 g
343-08245
Separating Gel Buffer Solution (x4)
250 mL
192-11041
Ready-to-Use mixed solution of "Sol. Band "Sol. Dfor preparation of Resolving Gel. Contains SDS.
Stacking Gel Buffer Solution (x4)
250 mL
199-11051
Ready-to-Use mixed solution of "Sol.Cand "Sol. Dfor preparation of Stacking Gel. Contains SDS.
N,N,N',N'-Tetramethylethylenediamine (TEMED)
25 mL
205-06313
for Electrophoresis
10 w/v% Ammonium Peroxodisulfate Solution
             (Ammonium Persulfate Solution)
25 mL
019-15922
Ready-to-Use "Solution G"
◆Premixed Buffers
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
Running Buffer Solution (x10)
1 L
184-01291
Ready-to-Use concentrated "Solution H"
SDS-PAGE 10x Running Buffer
1 L
312-90321
Ready-to-Use concentrated "Solution H"
5 L
318-90323
SDS-PAGE Buffer, pH 8.5
5 L
192-16801
Ready-to-Use "Solution H", 1×buffer.
Tricine Running Buffer Solution (×10)
1 L
200-17071
Composition: 0.5 M Tris/0.5 M Tricine/1% SDS
Sample Buffer Solution (2ME+) (×2)
25 mL
196-11022
Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol
Sample Buffer Solution
             with 3-Mercapto-1,2-propanediol (×2)
25 mL
196-16142
Laemmli Sample Buffer containing   3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME
AquaBlot 10×High Efficiency Transfer Buffer
1 L
015-26213

Can use it to transfer to a
menbrane with high efficience.
◆Enzyme for Dephosphorylation
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
Alkaline Phosphatase(for   Biochemistry)
50 U
018-10693
Applicable to dephosphorylation of proteins
100 U
012-10691
◆Reagents for Staining
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
Quick CBB Plus
250 mL
174-00553
Ready-to-Use Sol. K. Fixing and destaining procedure are not required.
           No organic solvents are necessary.
           Protein bands are stained in 10 minutes.
1 L
178-00551
Quick-CBB
             ?Staining solution A: 1L x 1
             ?Staining solution B: 1L x 1
2 L
299-50101
By mixing staing solution A and B,   ready-to-Use Sol. K
Silver Stain MS Kit
20 tests
299-58901
Modified proteins by glycosilation and/or phosphorylation can be detected sub-nanogram level on the electrophoretic gel.
Silver Stain Kit Wako
for 10 gels
299-13841
50~100 times more sensitive than CBB method.
Silver Stain Ⅱ Kit Wako
for 10 gels
291-50301
This kit contains Stopper, which can be adjusted the staining strength.
Negative Gel Stain MS Kit
20 tests
293-57701
Applicable to mass analysis and Western blot
◆Protein Size Marker
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
WIDE-VIEW Prestained Protein Size Marker Ⅲ
25 μL
236-02463
A recommendable prestained marker used with Phos-tag SDS-PAGE because obtained bands are less distorted.
500 μL
230-02461
500 μL x3
234-02464
◆Positive Control(for confirmation of separation capacity of Phos-tag SDS-PAGE)
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
α-Casein, from BovineMilk, Dephosphorylated
1 mg
038-23221
Mixture of phosphylated and dephosphorylated α-casein
10 mg
034-23223
◆Electrophoresis Apparatus  Precast Gels
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
EasySeparator
1 unit
058-07681
An electrophoresis tank for SuperSep precast polyacrylamide gels.
SuperSep Ace, 6%, 13 wells
10 gels
195-15171
Prior to use of SuperSep Phos-tag PAGE, please use these as sample confirmation.
           Expired in 9 months after manufacture
SuperSep Ace, 7.5%, 13 wells
10 gels
198-14941
SuperSep Ace, 7.5%, 17 wells
10 gels
191-14931
SuperSep Ace, 10%, 13 wells
10 gels
195-14951
SuperSep Ace, 10%, 17 wells
10 gels
192-14961
SuperSep Ace, 12.5%, 13 wells
10 gels
199-14971
SuperSep Ace, 12.5%, 17 wells
10 gels
196-14981
SuperSep Ace, 15%, 13 wells
10 gels
193-14991
SuperSep Ace, 15%, 17 wells
10 gels
190-15001
◆Reagents for Western Blotting
Product Name
Pkg. Size
Wako
Cat. No.
Use Application
ImmunoStar LD*
           ?Luminescence solution A
           ?Luminescence solution B
200 cm2
296-69901
Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate.
           Not available for sale in the US and Europe.
1,000 cm2
292-69903
2,000 cm2
290-69904
ImmunoStar Zeta*
200 cm2
291-72401
Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal.
1,000 cm2
297-72403
2,000 cm2
295-72404
ImmunoStar Basic*
200 cm2
295-75101
A cost-effective, stable and long-lasting product.
2,000 cm2
291-75103
5,000 cm2
299-75104
Immuno Enhancer
2 assays
294-68601
Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA
10 assays
290-68603
40 assays
298-68604
* : Not available for sales in the US and Europe.

 

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